The latest research looking into the complexities of termite nest structure, swarming behaviour and the roles of the royal pair and reproductives.
Latest termite nest and reproduction research
Termite mounds – designed for strength and water management
From observation, pest managers will know how hard the external layer of a termite mound can be and we also know that termites are particularly sensitive to dehydration. Researchers in Thailand have taken a closer look at the mound structures of Globitermes sulphureus, a common termite found in savannah environments.1 In such locations it is exposed to both hot temperatures (and therefore the risk of dehydration) and heavy rains and potential flooding events. A range of tests were carried out on the three layers that make up the structure of the termite mound to determine their functional role, assessing the water permeability, water retention and resistance to raindrop impact, whilst also measuring humidity within the mound.
Based on their physical attributes, the researchers concluded that the outer layer is not only physically strong but reduces water loss from the nest. The middle layer is distinguished by its resistance to water infiltration (heavy rain and flooding) and mechanical forces and is therefore integral to maintaining the structural integrity of the nest. The innermost layer had the ability to absorb and retain large amounts of water, but at the same time exhibited a slow rate of water loss, making it a critical water source for the termites and important in maintaining humidity. The termites appear to choose different materials to construct these layers, with soil and sand being key components in the outer two layers and fibrous, organic elements being key components of the innermost layer. The researchers believe that mound layers in structures made by other termite species will have similar functionality.
How do termites know how to build mounds?
Whilst the knowledge about termite mound structures and their physical attributes is increasing, there is actually very little information on what stimuli are being used to guide the mound construction decisions and how these are detected by the termites. Previously, various factors have been suggested to explain pellet deposition, the key act in construction. These have included elevation, digging activity, humidity transitions, surface curvature and even the presence of a construction pheromone. One of the key challenges in carrying out research in this area is that termites often dig and build in the same area, making it difficult to separate the stimuli that trigger the digging and building behaviours.
Using an elaborate series of laboratory trials with prefabricated foundation constructions and pellets, researchers demonstrated that termites deposited pellets on curved surfaces, more specifically at locations that also demonstrated a moisture differential.2 At a basic level this makes sense; a barrier should be built at a location where there is evaporation (loss of water) thus by building a barrier the termites can maintain internal nest humidity. However, although this study specifies the stimulus for the simple building rule for pellet placement, more studies are required to elucidate how this rule impacts the more complex construction of entire mounds and how it may vary between species.
Termites secrete anti-fungal compounds at foraging sites
Soil and rotting wood are environments with a high microbe loading (fungi and bacteria). Successful termite colonies need to be kept free of pathogenic microbes and parasites, so it would make sense for foraging workers to take action to minimise the introduction of pathogens on foraging material. A recent study has determined that foraging workers of Reticulitermes speratus secrete phenylacetic acid, which is known to have anti-fungal properties.3 The study demonstrated that phenylacetic acid inhibits germination and/or mycelial growth of key pathogenic fungi. The researchers calculated that the amount of phenylacetic acid secreted by a single worker in 24 hours would be sufficient to suppress this fungal growth. Therefore, at foraging sites with a significant number of workers, the level of phenylacetic acid would be sufficient to suppress fungal growth. As workers also secrete phenylacetic acid onto nest material, it provides anti-fungal benefits both inside and outside the nest.
How do fungus-growing termites keep fungal pathogens under control?
While generic anti-fungal secretions help non-termitid subterranean termites, fungus-growing termitids, such as Odontotermes obesus, have a more challenging situation. They need to keep their nest clear of pathogenic fungus whilst protecting their symbiotic Termitomyces fungus. Through a series of trials – isolating some 418 different fungal genera from the termite’s environment, termite workers and fungal combs (as they go through stages of decay) – researchers proposed a complex, multi-organism relationship within the fungal gardens of Odontotermes obesus.4
Termitomyces is under continuous threat from pathogenic fungi brought into the nest on the bodies of unsuspecting workers. Termitomyces is capable of inhibiting the growth of several invasive fungi, but not Pseudoxylaria. Pseudoxylaria itself had inhibitory effects against almost all the fungi tested and it is omnipresent in all nests. However, if allowed to germinate, it proliferates after 24 hours, and the termites probably have less than 48 hours to remove the Pseudoxylaria before it takes over the fungus garden. The authors of the study therefore suggest that although the termites can only feed on Termitomyces, it is the combination of Termitomyces and Pseudoxylaria that keeps deleterious fungi at bay, and it is a combination of termite weeding and the presence of symbiotic bacteria that are active against Pseudoxylaria that keeps its growth in check. Further study and data are required to confirm the various aspects of this hypothesis.
Queens and worker termites have different strategies for fungal protection
Keeping pathogenic fungi out of the nest is vital forcolony health, but with the queen being a more important member of the colony, the question is whether there are any different protection mechanisms in place for the queen compared to workers? Researchers investigated this question looking at how Reticulitermes chinensis responds to the fungus Metarhizium anisopliae.5 Infected queens received significantly more grooming time from clean nestmates than did infected workers, but returned much less grooming time to clean nestmates than did infected workers. Infection also initiated differences in gene expression, with upregulated expression of a number of genes involved in immunity in the queens, but not in the workers.
Alate co-ordination for swarming flights
It is generally accepted that synchronised swarming in termites, both within a colony and between colonies of the same species in the same area, is triggered by specific environmental cues – a combination of temperature, humidity and recent rain events. However, recent research has shown this isn’t necessarily the case. Working with Reticulitermes kanmonensis, in laboratory studies under semi-field conditions with fluctuating temperatures, alates within the same colony synchronised their dispersal flight at warmer temperatures, with flights suppressed at lower temperatures.6 However, even for colonies held at a constant 20ºC, dispersal flights were synchronised, indicating environmental cues cannot be the only triggers for synchronisation of mating flights. The researchers also observed that no matter the environmental regimes, more synchronisation occurred when there were larger numbers of alates present. They concluded that mating flights are therefore synchronised as a result of a combination of environmental and (as of yet) undetermined social factors.
How is the gut microbiota passed on to new alates?
In worker termites, the gut microbes are lost prior to each moulting. They repopulate their gut microbiota after moulting from their nestmates, through proctodeal trophallaxis. With a healthy gut microbiota essential for the establishment of a new colony, the question arises as to how alates have the necessary gut microbes if they are lost when they go through their final moult from nymph to adult (alate).
Working on Reticulitermes speratus in Japan, researchers established that nymphs retained their protists through their final moult to adult and that there was no proctodeal trophallaxis from workers post moult.7 However, the proportions of the various protist species were different between the two castes and the total number was lower in the alates than the workers. The authors hypothesise that the reduced quantity of protists helps reduce body weight for better dispersal and the differential composition may provide a better combination of protists to maintain optimal functioning in a newly founded colony. The research also observed that some of the protists show changes in morphology during the nymph to adult eclosion, presumably to provide protection against the significant changes in hindgut conditions during the moulting process.
References
1 Chiu, C.-, I. et al. (2024) ‘Three-layered functionally specialized nest structures enhance strength and water retention in mounds of Globitermes sulphureus (Blattodea: Termitidae)’, Environmental Entomology, 53(6), pp. 935–945. https://doi.org/10.1093/ee/nvae093
2 Facchini, G. et al. (2024) ‘Substrate evaporation drives collective construction in termites’, eLife, 12. https://doi.org/10.7554/eLife.86843
3 Nakashima, M. et al. (2024) ‘An antifungal compound secreted by termite workers, phenylacetic acid, inhibits the growth of both termite egg mimicking fungus and entomopathogenic fungi’, Insectes Sociaux, 71(2),pp. 221–232. https://doi.org/10.1007/s00040-024-00966-3
4 Agarwal, R. et al. (2024) ‘Investigation into how Odontotermes obesus maintains a predominantly Termitomyces monoculture in their fungus
combs suggests a potential partnership with both fungi and bacteria’, Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-06708-2
5 Dong, Y.-N. et al. (2024) ‘Different strategies between queens and workers against fungal pathogens in the termite Reticulitermes chinensis’, Ecosphere, 15(5). https://doi.org/10.1002/ecs2.4853
6 Mizumoto, N. and Nozaki, T. (2024) ‘The significance of social interactions in synchronized swarming flight in a termite’, Biology Letters, 20(11). https://doi.org/10.1098/rsbl.2024.0423
7 Inagaki, T. et al. (2024) ‘Transmission dynamics of symbiotic protist communities in the termite gut: association with host adult eclosion and dispersal’, Royal Society Open Science, 11(5). https://doi.org/10.1098/rsos.231527
Nest type impacts survival under dry conditions
Termites build nests underground, above ground (epigeal nests) and in trees (arboreal nests). These represent very different nesting and foraging environments. When it comes to managing moisture, on both an individual termite basis and within the nest overall, the desiccation stress increases as the nests move further above ground. Analysing the termites from different species of Termitidae with different nesting habits, researchers have demonstrated that termites from epigeal and arboreal nests exhibited lower rates of water loss than those from fully subterranean nests, which provides them with increased tolerance of drier conditions.1
Decreased cuticular permeability appears to be a key element of this increased tolerance. In addition, it was noted that termites from arboreal nests had a significantly higher water content. Not only would this protect against desiccation, but presumably provides termites with the capability to transport water to the arboreal nest.
Using queen size to estimate colony size
Accurately estimating colony size, apart from counting individual termites, is somewhat problematic, especially in the field. However, researchers working on laboratory colonies of Reticulitermes speratus have established that the weight of the queen (when measured at the peak of the egg-laying season), correlates strongly with egg production, which in turn correlates with the number of workers in the colony.2 The researchers then used the formula to calculate the sizes of colonies in the field, by collecting and weighing all the queens in each colony. The researchers believe such a method could be used in any species that has a clear seasonality (peak) in egg production. However, as this is essentially a destructive sampling method, it is not applicable to ongoing studies where regular monitoring of the same colony in the field is required.
Kings and queens move underground in winter
For termites that live in more temperate climates, where the winters can be cold, surviving the colder months is critical to success. Researchers in Japan studying Reticulitermes speratus found that the royals stayed above ground within decaying logs during the warmer months, but retreated to an underground royal chamber in the colder months.3 These underground chambers were typically in the roots of tree stumps, which were on average 3-4°C warmer than the logs above ground. The researchers also established that the kings and queens were also significantly more tolerant of the cold than the workers, which would obviously be beneficial in ensuring colony survival in extreme weather conditions.
Reticulitermes have the potential to produce hybrids too!
Whilst Coptotermes hybrids have dominated the scientific headlines over the last year or two (with concerns over the destructive potential of C. formosanus x C. gestroi hybrids) the phenomenon is not restricted to Coptotermes. Researchers evaluated the potential for hybridization between two sympatric Reticulitermes species, R. chinensis and R. flavipes.4 Despite these two termites being classified as two different species, laboratory trials showed that reproductives of both species readily mated with reproductives of the other species, with no difference in the frequency and duration of mating events. The cross-species pairing also produced viable offspring. Should these hybrid offspring be fertile, there is the potential to produce hybrid colonies in the field.
The authors hypothesise that the ease of such matings may improve the success of an individual achieving a successful pairing, given that pairing up is very much a chance event. Such pairings would provide the population with the benefit of gaining increased genetic diversity. However, it doesn’t necessarily mean that hybrid colonies become established in nature with the potential to create a new species. Back-crossing between hybrids and the pure-bred adults will result in gene dilution thus eliminating the possibility of hybrid speciation.
Factors affecting successful pair formation and colony founding
The success of paired alates in founding a colony is very much dependent on their own internal resources, namely body weight (stored fat) and gut symbionts. Researchers have found that body weight and protozoa numbers were higher in paired alates than single alates in Coptotermes formosanus.5 While it is possible that partners could select mates based on body size, it seems unlikely they could detect the protozoa composition. As such, the authors believe that rather than being selected for by one or other of the partners, the higher body weight and higher protozoa numbers provide dealates with the greater size and energy to outcompete rivals in the pairing process.
Both kings and queens pass on gut symbionts
Gut symbionts are integral to the success of non-termitid termites. Whilst it is often assumed that the queen contributes the gut symbionts to the first cohort in the new colony, with both a king and queen providing bi-parental care, this may be a misguided assumption. Looking at the protist communities of Coptotermes gestroi and C. formosanus alates and colonies, researchers established that individual alates generally harboured between 1-3 protist species, whereas colonies contained 4-5 protist species.6 The researchers hypothesised that both male and female alates contribute to the colony gut symbiont make-up.
Royal food for termite kings and queens
As reproductives, especially queens, have very different energy requirements, it is to be expected that they receive food of a different composition to workers. Working with Reticulitermes speratus, researchers have indeed shown this is the case, with kings and queens receiving different food to each other.7 It was further discovered that their gut morphology is also different to workers and to each other, in order to absorb the different nutrients. Amongst other differences, the queen-specific food contained nutrients required for egg development and the king-specific food contained nutrients required for sperm production.
The authors presume that worker termites produce these royal foods through secretions from their salivary glands. It would appear that workers have the ability to decide on food allocation to kings or queens, but this is the focus of future work – are individual workers capable of producing both royal foods or do kings and queens have a specific cohort of workers that provide their food?
Latest understanding on termite balls
Termite balls have been found in the nests of an increasing number of termite species. Often confused with termite eggs, they are actually sclerotia, a resting stage of a fungi, and contain a mass of mycelium and food reserves. Recent research has found that different sclerotia associate with different termite species, and that the termites will preferentially carry their related sclerotia, which the authors suggest is a species-specific interaction.8 The sclerotia are located amongst the eggs in the nursery and are tended in a similar way to the eggs, keeping them free of pathogens. It is thought the sclerotia benefit from these hygiene measures and the termites benefit from various fungal enzymes that may help with wood digestion.
References
1 Wanthathaen, C. et al. (2023). Desiccation tolerance of Termitidae termites in relation to their nest type. Environmental Entomology, 52(4):
555-564, https://doi.org/10.1093/ee/nvad066.
2 Takata, M. et al. (2023). A method for estimating colony size using queen fecundity in termites under field conditions. The Science of Nature, 110: 35.
3 Takata, M. et al. (2023). Discovery of an underground chamber to protect kings and queens during winter in temperate termites. Scientific Reports, 13: Article number 8809.
4 Wu, J. et al. (2023). Effect on genetic diversity of the absence of intraspecies preference in 2 sympatric Reticulitermes termite species
(Isoptera: Rhinotermitidae). Journal of Insect Science, 23(6)L 24; 1-7. https://doi.org/10.1093/jisesa/iead115
5 Chen, J. (2023). Weight and protozoa number but not bacteria diversity are associated with successful pair formation of dealates in the Formosan subterranean termite, Coptotermes formosanus. Plos One. https://doi.org/10.1371/journal.pone.0293813
6 Velenovsky, J.F. et al. (2023). Vertical transmission of cellulolytic protists in termites is imperfect, but sufficient, due to biparental transmission. Symbiosis 90: 25–38.
7 Tasaki, E. et al. (2023). The royal food of termites shows king and queen specificity. PNAS Nexus, 2(7), https://doi.org/10.1093/pnasnexus/pgad222
8 Costa-Leonardo, A.M. (2024). New reports on the association between eggs and sclerotium-forming fungi in Neotropical termites with insights into this mutualistic interaction. Biological Journal of the Linnean Society, blae010. https://doi.org/10.1093/biolinnean/blae010
Life and death of a termite colony
The life cycle of a termite colony is often perceived as a relatively straightforward development pathway: colony founding, growth to maturity, maturity, senescence (old age) and then death. However, recent analysis of a massive field-collected data set, from studies on Coptotermes formosanus over a 25-year period (1985- 2009), suggests there is much greater variability in colony reproduction, demographics and longevity even within a termite species.1
The study describes the changes in the colony dynamics of four colonies over their lifetime. Certainly, one colony followed the ‘standard’ development pathway and was active for a ten-year period, then declined for three years before death. However, other colonies had more variable productivity, responding to the death/fecundity of the primary reproductives in different ways and also potentially being influenced by external factors, such as food availability, climate and competition.
The ability to produce secondary reproductives (which doesn’t always occur) is critical to surviving the loss of the primary reproductives and can allow a colony to thrive for 20-30 years. The second study nest had a pause in reproductive output, which was only rejuvenated following the production of secondary reproductives. The third colony showed a similar pattern although produced a large number of secondary reproductives with the extensive cycles of inbreeding producing smaller individuals across castes. The fourth study nest showed a massive nymph production before senescence, followed by a successful colony takeover by a neighbouring young colony. The ‘reborn’ colony itself was then highly productive for ten years before losing production and then recovering with the production of secondary reproductives. There is no one-size-fits-all when it comes to the life cycle of a termite colony.
Choosing a replacement queen
Many termite species produce secondary reproductives that replace the primary queen should she die. But how does the colony choose which termite workers should develop into reproductives? Some recent research on looked at the behaviours of workers before they differentiated into reproductives. They identified that the workers that developed into reproductives moved less, performed more proctodeal trophallaxis, and were groomed more than others.2 The researchers suggested that the reduced mobility would conserve energy, and the anal feeding and increased grooming would indicate more dominant status. This provides some information on how the colony supports the development of individual workers into secondary reproductives but doesn’t provide information on why they were chosen in the first place.
The same researchers also noticed that when R. labralis workers encountered a closely related species, R. aculabialis, they were either aggressive or ran away. They also noticed that when neotenics of R. labralis were introduced to groups of R. aculabialis they always displayed signs of retreat and avoidance (“elusive” behaviour).3 The researchers went on to demonstrate that “elusive” workers were more likely to develop into reproductives and do so faster than “aggressive” workers. The researchers concluded that with the aggressive trait being a useful behaviour for colony defence, it would make evolutionary sense for the more elusive workers, which show high self-preservation behaviours, to develop into reproductives. They did not investigate whether there was any differences in the sex of the reproductive that developed from so-called elusive or aggressive workers.
Queen primer pheromones
The queens of social insects, in particular ants, wasps and bees, use pheromones – the queen primary pheromones (QPP) – to maintain their reproductive dominance. Although it is believed to be the same in termites, to date the QPP has only been identified in one species of lower termite, Reticulitermes speratus, a volatile blend of 2-methyl-1-butanol and n-butyl n-butyrate. Recent research has now identified the first QPP in a higher termite (Termitidae), Embiratermes neotenicus. In this species, the primary queen is replaced by neotenics produced through parthenogenesis (asexual reproduction).4 Researchers identified the sesquiterpene (3R,6E)-nerolidol as the QPP in E. neotenicus and noted that it supresses the development of parthenogenic neotenic queens i.e. when the primary queen was present and healthy, neotenic production would be suppressed.
‘Termite balls’ and termite eggs
Within some species of subterranean termite (Rhinotermitidae), a fungus is found inside the colony nursery mixed in with the termite eggs. These eggmimicking fungi are referred to as ‘termite balls’. They range in colour from light to dark brown and are between 0.2-0.35 mm in diameter. It is not clear whether this is a parasitic relationship (fungus receiving all the benefit) or a mutualistic relationship; however it has been suggested that the fungus gains protection inside the nest and the termites potentially benefit from using the fungus as a food or as source of cellulase enzymes to aid with wood digestion. These ‘termite balls’ have been reported in seven Reticulitermes species, Coptotermes formosanus and most recently Coptotermes gestroi.5
References
1 Chouvenc, Thomas & Ban, Paul & Su, Nan-Yao. (2022). Life and Death of Termite Colonies, a Decades-Long Age Demography Perspective. Frontiers in Ecology and Evolution. 10. 911042. 10.3389/fevo.2022.911042.
2 Bai, Zhuang-Dong & Liu, Yibin & Sillam-Dussès, David & Wang, Rui-Wu. (2022). Behavioral differentiation among workers may reduce reproductive conflicts during colony inheritance in the termite Reticulitermes labralis. Insectes Sociaux. 69. 10.1007/s00040-022-00862-8.
3 Bai, Zhuang-Dong & Shi, Chong-Yang & Sillam-Dussès, David & Wang, Rui- Wu. (2022). Elusive workers are more likely to differentiate into replacement reproductives than aggressive workers in a lower termite. Current Zoology. 69. 10.1093/cz/zoac040.
4 Dolejšová, Klára & Křivánek, Jan & Štáfková, Jitka & Horáček, Natan & Havlíčková, Jana & Roy, Virginie & Kalinova, Blanka & Roy, Amit & Kyjaková, Pavlína & Hanus, Robert. (2022). Identification of a queen primer pheromone in higher termites. Communications Biology.5. 10.1038/s42003-022-04163-5.
5 Costa-Leonardo, Ana & Janei, Vanelize & da Silva, Iago. (2022). First Neotropical record of the association between brown sclerotium-forming fungi and termite eggs in a nest of Coptotermes gestroi (Blattaria, Isoptera, Rhinotermitidae). The Science of Nature. 109. 10.1007/s00114-022-01815-8.
Predicting termite mound structures
Researchers using computer modelling to predict termite mound shape believe that in applying the principles of heat transfer and thermodynamics, computer-generated mound shapes closely match those found in the field.1 Essentially, sun and wind play an important role in the development of mound shape and structure.
Mounds exposed to more sun tend to have a cone-shaped structure that points towards the sun, whereas mounds in shaded areas tend to be vertical domes. Mounds in areas of increased wind are generally shorter in height. The sun and wind factors interact to determine the internal structure. When the need for thermoregulation is more important than the need for gas exchange, the mounds have thicker walls and are built deeper into the soil.
Termite swarms
Outside of tropical rainforests, termite alate flights often occur during a specific time of the year, typically during a warmer, more humid period. Whilst most pest managers will tell you this is the case, researchers in Brazil have confirmed the same whilst assessing alate flights in a seasonally dry tropical forest.2 Peak termite swarms, of all species, occurred during February and indeed 97% of all flights occurred during a 20-day period.
The number of flight occurrences and species composition were significantly influenced by the accumulated precipitation over 72 hours and air density. This high level of synchronisation between species suggests that in environments which have variable moisture levels throughout the year, termites have evolved to swarm when conditions provide the best chance of survival.
References
1 Fagundes, Tadeu & Ordonez, J. & Yaghoobian, Neda. (2021). The Role of Mound Functions and Local Environment in the Diversity of Termite Mound Structures. Journal of Theoretical Biology. 527. 110823. 10.1016/ j.jtbi.2021.110823.
2 Lucena, Emanuelly & Silva, Israel Soares & Monteiro, Sara & Moura, Flávia & Vasconcellos, Alexandre. (2021). Accumulated precipitation and air density are linked to termite (Blattodea) flight synchronism in a Seasonally Dry Tropical Forest in north-eastern Brazil. Austral Entomology. 61. 10.1111/aen.12577.
How many termite reproductives are in a nest?
For pest managers carrying out termite work, the holy grail is to find the termite queen (if they are able to find the nest). Many would assume that the termite colony comprises a ‘simple family’ – a single pair of primary reproductives, which mate monogamously. This is the most common situation, but it isn’t always the case.
Termites create two other types of colony structure. Firstly, there is the extended family where one or both of the founding pair are supported or have been replaced by secondary reproductives that have been produced from within the colony. Extended family colonies appear to be more likely in lower termites (60%) compared to higher termites (13%).1
Secondly, there are ‘mixed family’ colonies, which can arise through three different mechanisms: when two dispersing alates found a colony together; from fusion between two simple families; or through adoption of alates.
Researchers in Australia utilised DNA analysis to investigate the prevalence of the different colony types in Nasutitermes exitiosus.
Analysing 60 colonies from across NSW and ACT, the researchers determined that 61.7% were simple colonies, 16.7% were extended families and 21.7% were mixed families. Most of the mixed-family colonies contained two queens and one or two kings. In one colony, the DNA evidence suggested there were seven primary queens. How such mixed family colonies are formed is unclear, with the researchers suggesting that future investigations should focus on the potential impact of local ecological and climatic conditions on breeding patterns and colony structure.
Long live the Queen (and King)
As a general rule in animals there is a trade-off between longevity and reproductive output – you can be either long lived or have a high reproductive output, but you cannot have both. The exception to the rule are social insects, especially the termite queens and kings, which can live for 20 years or more.
In their review on the subject, Tasaki et al. (2021)2 point out that a range of external factors rather than ageing per se impact life expectancy – predation, starvation and adverse weather events will all cause death. However, in a colony situation, the termite reproductives that remain safe in the centre of the colony and are protected and fed through the activity of other colony members are largely insulated from these mortality factors. Thus the development of eusociality with the subsequent removal of these pressures on the reproductives is one of the key reasons why they live so long.
However, there must also be a physiological and molecular basis to the longevity; there must be a delay in the ageing process that is normally seen in other animals. There is evidence to suggest that the antioxidant and DNA repair systems are augmented to slow the age-associated accumulation of physiological damage. An alternative theory (not mutually exclusive) is that reproductives have a lower expression of growth signalling pathways, which prevents the reduction in gene expression that occurs later in life.
Another factor that may contribute to their longevity is the hypoxic nature of the royal chamber. Deep in the nest, the oxygen levels are low (15%) and CO2 levels are high (5%). In the atmosphere, oxygen content is around 21% and CO2 levels are less than 0.05%. Clearly, reproductives have had to adapt to this very different atmosphere inside the nest and indeed queen egg-laying increases greatly under these nest conditions, compared to normal atmospheric conditions.3 These lower levels of oxygen would reduce oxidative stress on reproductives (oxygen can be toxic) and the development of anaerobic energy producing systems in these low-oxygen environments may also contribute to their long lifespans, but this needs to be investigated further.
References
1 Montagu, A., Lee, T.R.C., Ujvari, B., McCarl, V., Evans, T.A., Lo, N., 2020. High numbers of unrelated reproductives in the Australian `higher’ termite Nasutitermes exitiosus (Blattodea: Termitidae). INSECTES SOCIAUX 67, 281–294. https://doi.org/10.1007/s00040-020-00764-7
2 Tasaki, E., Takata, M., Matsuura, K., 2021. Why and how do termite kings and queens live so long? PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 376. https://doi.org/10.1098/ rstb.2019.0740
3 Tasaki, E., Komagata, Y., Inagaki, T., Matsuura, K., 2020. Reproduction deep inside wood: a low O-2 and high CO2 environment promotes egg production by termite queens. BIOLOGY LETTERS 16. https://doi.org/10.1098/rsbl.2020.0049
Further reading:
All female termite colonies discovered and understanding how they evolved.
How queen termites choose their mates.
Termite queens can live for over 20 years. Here’s the latest research on queen longevity and queen succession in termite colonies.
How termites start a termite mound.
How do termites build their mounds and why are they the shapes they are?
Termite soldiers are the muscle of the colony but they are also important in termite nest hygiene.
